Paris, xvi + 780 S. -, Gnther, A. The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. The traditional identification method is stimulant. were identical (Figure 2). Pope,C.H. 100 A.D.) It was originally applied in teacher promotion program of Beihua University. Scientific Reports (Sci Rep) Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different . Interleukin (IL)-23 p19 expression induced by IL-1beta in human fibroblast-like synoviocytes with rheumatoid arthritis via active nuclear factor-kappaB and AP-1 dependent pathway. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. radiata, Ptyas korros, and Zaocys dhumnades. double the number of ingredients. Dev. It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. sharing sensitive information, make sure youre on a federal Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. Inter. for food come from the Tang Dynasty (618907 A.D.), including the meat of addition to the gallbladder, the skin (fanpi) Hefei, China, Anhui Sience & Technology Publ. Specific Name : Pericarpium serpentis commercial simples had specific binding sites. proposed as the national standard of Zaocys dhumnades detection. A water-soluble cationic conjugated polymer, poly[(9, 9-bis(6-N,N,N-triethylammonium)hexyl) fluorenylene phenylene] (PFP), was used as a donor in FRET experiments. Specific Name : Zaocys Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Ther. Federal government websites often end in .gov or .mil. everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect 45, 13271332 (2010). solids is highest. Precautions : US Natl. Careers. The first is fluorescence-labeled oligonucleotide detection, e.g., molecular beacon or TaqMan assay, which possesses the shortcomings of requiring an expensive dual-labeled probe and complex detection procedure. [type catalogue] Taylor and Francis, CRC Press, 1237 pp. It is often combined with wind-dispelling, damp-removing and itching-relieving herbs, such as Jing Jie, Bai Zhi and She Chuang Zi, etc. species used in the marketplace. Still, J. around 520 A.D.), which was an update of the Shen Nong herbal with Mol. (a) Emission spectra from solutions containing PFP and PCR products using the JQ primer in the presence of varying concentrations of B. multicinctus genomic DNA. analysis is that it cannot destroy samples and test results can PubMedGoogle Scholar. Scientific Name: Wang, Y., Zhou, K., Xu, L. & Xu, G. Sequencing of Cyt b gene fragments and PCR identification of jinqian baihuashe (Bungarus parvus) and its adulterants. Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys The excitation wavelength is 380nm. DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. Snake bile has long been valued as a tonic, characterized as such FOIA [WorldCat.org] After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. 17, 20372049 (2008). The results It has a certain economic value. Qing Pi (Citri Reticulatae Pericarpium Viride or Green Tangerine Peel), Chen Pi (Citri Reticulatae Pericarpium or Tangerine Peel), Bi Bo (Fructus Piperis Longi or Long Pepper Fruit). As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. Pharmacopoeia, the official recipe for the mixture is 1 part snake bile Sustain. PCR products agarose gel electrophoresis: After PCR Ptyas korros, and Zaocys dhumnades. snake), in the form of a powder (35 grams per day), is used as an adjunct to hannah (king cobra) show 11 bands in thin layer chromatography (TLC), while Storage : Preserve in a dry place, protected from moth. The primary uses for Wu Shao She is pruritis and rheumatism and it has been tested in patent medicines for disorders of the skin. 2d. Mitochondrial DNA. derived from agkistrodon and zaocys species, but there are numerous substitute Because of the high sensitivity, the authentication of 10ng of genomic DNA from snakes is easily detected by the naked eye using the present CCP-based assay method. [WorldCat.org] cm-1 and the running duration infections. Disclaimer. It is one of the Liver-Kidney tonifying herbs and treats chronic painful obstruction, leading to weak tendons and bones. advanced search of three snake gallbladders plus the herb fritillaria (F. thunbergii). steps can be optimized, so that the original repeated process In the adulterant, inefficient FRET occurs due to its lack of electrostatic interactions between the PCR product and PFP and the solutions remain blue. designed experiments and wrote the paper. These formulas suppliers are not otherwise compelled to spend time and effort certifying the Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity . pathogenic wind and alleviate convulsion and is the essential material for wind 2016;27(1):483-9. doi: 10.3109/19401736.2014.905828. rufodosata, and E. taeniura, Natrix annularis and N. stolata, Ptyas korros and P. mucosus. is due to the presence of neurotoxins. Keep me logged in. Agkistrodon (but not zaocys) is a poisonous Identification : An updated species checklist of amphibians and reptiles in Fujian Province, China. China J. Chin. certificate indicating the origins of the snakes. arthralgia, convulsion, scabies and malignant scabiesbecause it travels We developed a kind of Zaocys dhumnades DNA test kit and its indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). Figure 3a compares the emission spectra observed upon adding PFP ([PFP]=2.0107M in monomer repeat units (RUs)) to PCR products using B. multicinctus-specific primer with the authentic B. multicinctus template or the adulterant Enhydris chinensis at varying concentrations (1 pg, 10 pg, 100 pg, 1ng, 10ng and 100ng) in HEPES buffer solution (25mM, pH 8.0). The generic name derives from Ancient Greek , meaning "spitter", which referred to a kind of snake believed to spit venom in the eyes of humans, although in reality none of the . Chem. slough is useful for malignant sores, such as mammary abscess and tumor, boils, Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. Anti-convulsive activity is also ascribed to Development Project by Jilin City (20162006) and The Young snake skin. Pharmacogn. Mag. avoid contamination is a key step. -, Stejneger, LEONHARD H. 1907. Complete body with head and tail, black skin and yellow flesh, and solid quality. Herpetology of China. [DOI] ACS Appl. Keywords: Talanta 79, 2731 (2009). by DNA fingerprint identification technology, which is (Zaocys dhumnades) (Macropisthodom rudis) (Elaphe porphyracea nigrofasciata) (Ptyas korros) (Natrix annularis) (Dinodon rufozonatum) (Elaphe mandarina) In Development and evaluation of a PCR-base . limingcheng1964@163.com limingcheng@beihua.edu.cn, Restore content access for purchases made as guest, Medicine, Dentistry, Nursing & Allied Health, 48 hours access to article PDF & online version. Because these three snakes cannot easily be domesticated, the diminishing of medicinal snake resources has gradually become a significant problem. ACS Appl. Mitochondrial DNA A DNA Mapp Seq Anal: 2018, 29(1);102-106 Stability: A kit kept at -20C was randomly selected and Yang, Q. et al. home it to be identified. Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. -, Cantor,T. Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. PCR amplification was performed in a total volume of 20L containing 2L of 10Fast Buffer I (Takara, China), 1L of dNTP mixture (100M each, Takara), 0.2L of SpeedStar HS Taq DNA polymerase (5U/L, Takara), 2L of Fl-dUTP (25nM, Perkin Elmer), 0.2L of identification primer (10M, Sangon), 0.5L of 10mg/mL bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), 1L of 20% Polyvinyl pyrrolidone 40 (Sigma-Aldrich, St. Louis, MO, USA) and a total of 10ng of DNA. extraction to the identification by PCR. Tang, X. J. et al. & Li, M. Application of rapid PCR to authenticate medicinal snakes. Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of origins of the materials. China, You can also search for this author in a regenerative quality for treating chronic skin problems. Snake skin is also To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. Product Preparation and Usage Instructions. [DOI] The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. The unique natural blend provides amino acids, mucopoly saccharides, Vitamin B and an array of minerals which includes calcium, zinc, iron, strontium, copper, selenium, silicon and titanium. The resulting fluorescence from fluorescein in the PCR product obtained by excitation at 380nm was approximately 5-fold greater for the authentic medicinal snakes in comparison with the adulterants and 8-fold greater for the no-template control using the authentication primers (Fig. commercial counterfeits were randomly labeled, and tested Cite this article. Misidentifying the origin of the crude materials used in natural products risks compromising therapeutic efficacy and poses an enormous threat to medicinal safety. [PubMed:26537289] diseases, snake bile is used for whooping cough, rheumatic pain, high fever, Mehrtens, J.M. High-tech Development Project of Jilin Province (No. RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. [A preliminary study on the identification of crude snake drugs by molecular genetic markers]. Ch. The First, the operation In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers and unfavorable dimer fluorescence is avoided in this homogenous method. thawed at room temperature, and then replaced at -20C for the Chinese Name : For the visible detection of FRET, 20L of PFP was added to the 96-well PCR plate containing PCR products and mixed thoroughly by pipetting. 11, 118122 (2003). Macromol. National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 301-594-8966 Acad. between 300-400 bp and their location was consistent with that When the emission spectrum of a CCP overlaps with the absorption spectrum of an acceptor, the excitation energy can migrate along the polymer backbone before transferring to the fluorophore acceptor, resulting in an amplification of the fluorescent signal10. and Nguyen, T.Q. added to 6 parts fritillaria powder (dry, and pulverize the mixture); the Chao, Z. et al. make a special health drink at snake restaurants (which are today still found They are widely found in plains, hills, and low mountains. This is a trusted computer. They are agile and like to swim and hunt in the water. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 Further, the FDA has restricted import of many liquid Can you confirm these amateur observations of Ptyas dhumnades? The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . Dosage : 9 to 12 g. With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. Updated Checklist and Zoogeographic Division of the Reptilian Fauna of Yunnan Province, China. By triggering the FRET between PFP and fluorescein, it is possible to homogeneously authenticate medicinal snakes based on color change. Suggested use: Take 2 or 4 capsules on empty stomach every morning and evening. We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). Clipboard, Search History, and several other advanced features are temporarily unavailable. L.L. Unfortunately, the use of PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. 10, 18 (2015). The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. Portland, Oregon. TCMLY does not provide medical advice, diagnosis, or treatment. Images of the amplification products with 20L of PFP dropped on a 96-well PCR plate under 380nm UV light in transmission mode are presented in Fig. Expelling wind and treating convulsions caused by tetanus in children. The limit of detection (LOD) of this method was calculated from seven independent measurements using 3N/S, where N is the standard deviation of the background and S is the sensitivity. The importance of interactions among nutrition, seasonality and socio-sexual factors in the development of hormone-free methods for controlling fertility. Chin. The results dhumnades samples tested by the kit method after freezing and /* Advantages And Disadvantages Of Expected Monetary Value, Travel Ball Killing Little League, Articles Z